Elute 30 min 3 hours raditional spe phenomenex l web. The technique offers targeted selectivity for analytes of interest, versatility for a wide range of sample volumes, can be easily automated for highthroughput, and is performed by following quick and simple methods load. Phenomenex offers this spe users guide as a practical aide for spe method development, optimization, and troubleshooting. No manual or automated filtrate transfer steps required. Make your lab more productive with our free, easytouse tools. Solid phase extraction spe is a very targeted form of sample. Science unfiltered enabling scientists inside the lab. Pdf this paper focuses on the investigation of different types of spe sorbents. Learn how to develop a customized method for spe and view. Sample pretreatment for hplc spe nmr and hplcms analyses. Phenomenex offers this spe users guide as a practical aide for spe method. Free application and troubleshooting services are available to help you create optimized spe methods for your analysis global assistance phenomenex technical specialists are available to provide technical and product support worldwide contact phenomenex or your local phenomenex distributor for a complimentary guide or assistance. Solid phase extraction is an effective technique for cleaning up and concentrating samples, prior to hplc or gc analysis.
Strata silicabased solid phase extraction spe phenomenex. Intuitively we know more sorbent requires more solvent, less sorbent less solvent. The analytes of interest were then eluted with 4 ml of 0. Scribd is the worlds largest social reading and publishing site. Strataxa has been added to phenomenex s line of solid phase extraction spe products. Graphitized carbon black and how it will improve your lab. Sample preparation prepare the sample for analysis by acidifying the matrix to ph 2 using 50% h 2so 4 and adding 10% nacl. Engineering successful analytical methods using hilic as an alternative retention mechanism overview. Ah06025 spe 12position glass chamber ea ah06027 spe cover, gasket and 12 stopcocks uea ah06029 spe gaskets 2pk another ah06031 spe vacuum gauge, valve and glass chamber ea ah06037 spe collection rack, including shelves, legs, clips and posts ea ah06040 spe plate for mm test tube ea. Included in the base unit is the manifold format of choice 96well plates, 1 ml tubes, 3 ml tubes, or 6 ml tubes. It is the result of meticulous care given to the quality control of surface smoothness, pore structure and pore. Technical assistance free method development assistance, by the experienced clarity biosolutions team at phenomenex, is available for both lcms methodology and extraction.
Founder fasha mahjoor talks about how phenomenex came to be. However, it should be noted that in spe, free silanols can often be used to. Lower solvent consumption greater recoveries lower reagent consumption less sample handling less apparatus. Phenomenex hiring inside sales consultant chemistry in. For a theoretical discussion of spe, and chromatographic theory in general, we suggest that the reader consult the literature references 1. Solid phase extraction spe method development guide. Phenomenex s chromatography products advance the future of scientific analysis and investigation, ensuring the quality of essentials like your food, water, shampoo, and even cold medication. Strata silicabased solid phase extraction spe sorbents. Our unlikely story phenomenex hplc gc and spe youtube. Fast spe extraction of per and polyfluoroalkyl substances pfas pfas from milk, eggs, butter, cheese, and fish using quechers, spe, and lc msms. This spe method development tool will recommend an extraction method for you in under 1 minute. For a theoretical discussion of spe, and chromatographic theory in general, we suggest that the reader consult the literature references 14 in appendix iii. Phenomenex strives to be a reliable partner through quick responses to customer inquiries, rapid delivery of products, and complimentary technical references. Spe 100 l sample 1 ml sample final cleanup by spe contact phenomenex or your local phenomenex distributor for additional spe products and applications.
Mar 26, 2021 following extraction, 500ul of the acetonitrile phase was diluted with 15 ml of water and loaded onto a preconditioned, weak ionexchange spe tube phenomenex strataxaw 200 mg. The complete guide to solid phase extraction spe phenomenex. Our extensive quality control procedures provide trouble free solid phase extraction methods. A look at phenomenex from the customer perspective.
Glucuronidase enzyme hydrolysis to save on time, extra lab costs, and unnecessary transfer steps. Phenomenex technical consultants are available to help with your. Phenomenex uhplc, hplc, spe, gc leader in analytical. Smart digest ia kit, av nonmagnetic with sola spe and collection plate.
Pfas and genx from water by solid phase extraction and lc msms. Spe is a very targeted form of sample preparation that allows you to isolate your analyte of interest while removing. Pdf chemical investigation of sageretia thea by hplcspe. Strata sdbl, strata c8 and strata c18 from phenomenex were compared. The packed section has the same diameter like a 1 ml tube. Extraction of oligo therapeutics from biological samples. Silicabased sorbents loading capacities sample matrix sorbent mass blood, serum, plasma 50 mg sorbent.
A look into phenomenexs hplc column gc, column and sample preparation productsolid phase extraction, phospholipid removal. From drug discovery and pharmaceutical development to disease diagnosis, food safety to environmental. Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, clinical. This technical tip will outline hydrophilic interaction chromatography or hydrophilic interaction liquid chromatography, hilic as an alternative retention mechanism that is particularly effective for lcms. Spe sorbent tips and syringes derivatizing reagents gc or hplc column focus liners for gc kit the ez. Free sample pack of solid phase extraction spe tubes use our spe method development tool to quickly develop an spe method and request your free stratax sample pack.
Free from interfering matrix elements at a concentration appropriate for detection or measurement. These manifolds permit convenient batch processing of spe or sample filtration and help ensure consistent extraction and filtration results. Science unfiltered enabling scientists inside the lab to. Phenomenex spe vacuum manifolds are available in 10, 12 and 24position configurations.
Phenomenex employees share insight into working for the chromatography company phenomenex. To start, enter the name of the analyte youd like to extract or select proprietary to develop a method based on the chemical characteristics of your analyte. Water load recommended volume of sample ph 67 see page 14. The highcapacity sorbent delivers maximum retention of anionic compounds, rounding out the companys complete offering for anioncation exchange separations. Phenomenex is a global manufacturer of uhplc, hplc columns, gc columns, sample preparation products and chromatography accessories and consumables. Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, clinical research, governmental and academic laboratories. Pfas analysis in water samples using lcmsms large volume direct injection. Pdf development and optimization of the spe procedure for. The first fritless spe product range which has been specifically designed. Spe reference manual and user guide phenomenex uhplc.
Your introduction to solid phase extraction, how it works, and how to get started. In the following application note we outline a simple approach for solid phase extraction method development using strata and stratax spe sorbents. The complete guide to solid phase extracton spe net. Ah06025 spe 12position glass chamber ea ah06027 spe cover, gasket and 12 stopcocks uea ah06029 spe gaskets 2pk another ah06031 spe vacuum gauge, valve and glass chamber ea ah06037 spe collection rack, including shelves, legs, clips and posts ea ah06040 spe. If stratax spe products do not perform as well or better than your current spe product of similar phase, mass and size, send in your comparative data within 45 days and keep the stratax spe product for free. Phenomenex s strata spe products are designed to meet all of your sample preparation needs. Phenomenex has built an intellectual property portfolio of patents, trade secrets, and innovations covering a wide range of enabling technologies, which include surface chemistry, polymer and silica particle technology, proficiency testing standards, as well as column hardware systems and novel packing technology. Empore spe disk envidisk spe disk spe particles embedded in glass fiber matrix available in c18 and c8 better flow properties but more resistant to clogging more prone to fines empore spe disk spe particles embedded in a ptfe fiber matrix available in over 9 x different chemistries. Phenomenex solid phase extraction method development software instantly develops analyte specific spe methods on stratax sorbents generates methods for traditional and highthroughput screening provides fast, accurate, and simple spe method development for the latest updates in spe method development, technical.
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